Read ebook - Series in Cellular and Clinical Imaging: Optical Probes in Biology 6 (2015, Hardcover) in DJV, FB2, DOC
9781466510111 1466510110 Optical probes, particularly the fluorescent varieties, enable researchers to observe cellular events in real time and with great spatial resolution. Optical Probes in Biology explores the diverse capabilities of these powerful and versatile tools and presents various approaches used to design, develop, and implement them. The book examines the use of optical probes to detect and track numerous molecular processes in living cells, including GTPase and kinase activities, membrane lipids, voltage, metal ions, metabolic signals, RNA, and histone modifications. It critically reviews the different probe designs and delves into the strategies for developing new fluorescent protein varieties with enhanced capabilities. It also covers sophisticated imaging techniques and equipment, such as intensity and lifetime-based fluorescence microscopy methods, used to visualize and track optical probes. In addition, the book goes beyond live-cell tracking to discuss the growing application of activity-based probes for performing pharmacological drug screening and probing molecular processes in living animals. It also discusses emerging techniques that are expanding optical probe-based approaches into new biological frontiers. With contributions from top international scientists, this book offers a thorough overview of the latest optical probes in cell biology and biochemistry. Both newcomers and established researchers will discover how to incorporate state-of-the-art optical probes and fluorescence imaging into their research., This book highlights the key concepts underlying design and application of optical biosensors in diverse contexts. It begins with an historical perspective on the field, moving on to foundations of engineering biosensors and development of fluorescent imaging approaches. The core chapters focus on the design and application of specific optical biosensors in vivo, either for tracking certain biomolecules or visualizing certain cellular events in various living systems. The final section addresses several additional applications, including use in high throughput drug screening and in integrated methods that involve modeling or optogenetics.
9781466510111 1466510110 Optical probes, particularly the fluorescent varieties, enable researchers to observe cellular events in real time and with great spatial resolution. Optical Probes in Biology explores the diverse capabilities of these powerful and versatile tools and presents various approaches used to design, develop, and implement them. The book examines the use of optical probes to detect and track numerous molecular processes in living cells, including GTPase and kinase activities, membrane lipids, voltage, metal ions, metabolic signals, RNA, and histone modifications. It critically reviews the different probe designs and delves into the strategies for developing new fluorescent protein varieties with enhanced capabilities. It also covers sophisticated imaging techniques and equipment, such as intensity and lifetime-based fluorescence microscopy methods, used to visualize and track optical probes. In addition, the book goes beyond live-cell tracking to discuss the growing application of activity-based probes for performing pharmacological drug screening and probing molecular processes in living animals. It also discusses emerging techniques that are expanding optical probe-based approaches into new biological frontiers. With contributions from top international scientists, this book offers a thorough overview of the latest optical probes in cell biology and biochemistry. Both newcomers and established researchers will discover how to incorporate state-of-the-art optical probes and fluorescence imaging into their research., This book highlights the key concepts underlying design and application of optical biosensors in diverse contexts. It begins with an historical perspective on the field, moving on to foundations of engineering biosensors and development of fluorescent imaging approaches. The core chapters focus on the design and application of specific optical biosensors in vivo, either for tracking certain biomolecules or visualizing certain cellular events in various living systems. The final section addresses several additional applications, including use in high throughput drug screening and in integrated methods that involve modeling or optogenetics.